Description
Immortalized Human Skeletal Muscle Myoblasts-SV40T from Creative Bioarray were developed from human skeletal myoblasts isolated from human adult tissue transduced with a lentiviral expression vector containing the SV40T gene. The cells show the typical fibroblast morphology. The cell line was continuously cultured for more than 50 population doublings without showing signs of growth retardation or replicative senescence.
Recommended Medium
SuperCult® Immortalized Human Skeletal Muscle Myoblast Medium (Cat No.: CM-I9102L)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Immortalization Method
SV40 large T antigen
Applications
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Recommended Products
CSC-7713W Human Skeletal Muscle Myoblasts (HSkMM)
CIK-HT013 HT® Lenti-hTERT Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
What documentation or support is provided with the purchase?
Each order includes a Certificate of Analysis, detailed usage instructions, and a culture manual. Additionally, our experienced technical support team is available to assist with any questions or challenges that may arise during your research.
Can Immortalized Human Skeletal Muscle Myoblasts-SV40T differentiate into myotubes?
Yes, Immortalized Human Skeletal Muscle Myoblasts-SV40T are capable of differentiating into myotubes. This process requires the use of a specialized differentiation medium, which is available for purchase separately.