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Immortalized Human Renal Proximal Tubule Cells-hTERT
Cat.No.: CSC-I0721Z
Species: homo sapiens
Source: Kidney
Cell Type: Epithelial Cells
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Cat.No.
CSC-I0721Z
Description
Immortalized Human Renal Proximal Tubule Cells-hTERT have been obtained immortalizing Human Renal Proximal Tubule Cells with Lenti-hTERT Lentivirus. Immortalized cells were controlled passaging side by side with the primary cells. Primary cells go into senescence after the 8th passage while the hTERT transduced cells go beyond 30 passages. This cell line is a great in vitro model system.
Species
homo sapiens
Source
Kidney
Recommended Medium
SuperCult® Immortalized Human Renal Proximal Tubule Cell Medium (Cat No.: CM-I2072Z)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Immortalization Method
Human telomerase reverse transcriptase (hTERT)
Applications
For Research Use Only
Cell Type
Epithelial Cells
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Quality Control
Real Time PCR was used to quantify hTERT gene expression in immortalized cell line.
free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations
free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations
Storage and Shipping
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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