Immortalized Human Pulmonary Microvascular Endothelial Cells

Cat.No.: CSC-I9128L

Species: homo sapiens

Source: Lung

Morphology: Polygonal

Culture Properties: Adherent

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Cat.No.

CSC-I9128L

Description

The pulmonary microvascular endothelial cells (PMEC) are isolated from human pulmonary arteries. The PMECs form a semiselective barrier that is critical for lung gas exchange and regulation of fluid and solute passage between the blood and interstitial compartments in the lung. HPAEC have been used for the study of vascular permeability and inflammatory responses. HPAEC in co-culture with HPASMC have been used as a model for pulmonary angiopathy. Immortalized Human Pulmonary Microvascular Endothelial Cells were derived from primary human tissue transduced with a lentiviral expression vector containing the SV40T gene. The cell line was continuously cultured for more than 20 passages without showing signs of growth retardation or replicative senescence.

Species

homo sapiens

Source

Lung

Recommended Medium

SuperCult® Immortalized Human Pulmonary Microvascular Endothelial Cell Medium (Cat No.: CM-I9128L)

Freezing Medium

Complete medium supplemented with 10% (v/v) DMSO

Culture Properties

Adherent

Morphology

Polygonal

Immortalization Method

SV40 large T antigen

Applications

For Research Use Only

Growth Properties

Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂.

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20 °C.

Shipping

Dry Ice.

Recommended Products

CSC-C1551 Human Pulmonary Microvascular Endothelial Cells
CIK-HT013 HT® Lenti-hTERT Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit

Quality Control

Real Time PCR was used to quantify SV40T gene expression in immortalized cell line. Free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations.

BioSafety Level

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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