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Immortalized Human Primary Trabecular Meshwork Cells-SV40

Cat.No.: CSC-I9168L

Species: Homo sapiens

Source: Juxtacanalicular and corneoscleral regions of human eye

Morphology: Polygonal

Culture Properties: Adherent

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Cat.No.
CSC-I9168L
Description
Immortalized Human Primary Trabecular Meshwork Cells-SV40 have been obtained immortalizing Human Primary Trabecular Meshwork Cells with Lenti-SV40 Lentivirus. Immortalized cells were controlled passaging side by side with the primary cells. Primary cells go into senescence after the 5th passage while the SV40 tranduced cells go beyond 20 passges.
Species
Homo sapiens
Source
Juxtacanalicular and corneoscleral regions of human eye
Recommended Medium
SuperCult® Immortalized Human Primary Trabecular Meshwork Cell Medium (Cat No.: CM-I2108Z)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
SV40 large T antigen
Applications
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Shipping
Dry Ice.
Quality Control
Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.
Storage and Shipping
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20°C.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
What are Immortalized Human Primary Trabecular Meshwork Cells-SV40?

These are human trabecular meshwork cells that have been genetically modified to proliferate using SV40 large T antigen. They provide a robust in vitro model for studying the biology of the trabecular meshwork and its role in ocular diseases.

What are the key research applications for Immortalized Human Primary Trabecular Meshwork Cells-SV40?

Immortalized Human Primary Trabecular Meshwork Cells-SV40 (Cat No.: CSC-I9168L) are ideal for studying eye disorders such as glaucoma, intraocular pressure regulation, aqueous humor outflow, and the trabecular meshwork's response to pharmaceutical agents.

How do these cells compare to primary trabecular meshwork cells?

Immortalized Human Primary Trabecular Meshwork Cells-SV40 (Cat No.: CSC-I9168L) offer a stable and consistent alternative to primary cells, which have a limited lifespan and variability. This allows for reproducible long-term studies and consistent data generation.

Are these cells suitable for drug testing and high-throughput screening?

Yes, their stable phenotype and extended lifespan make them suitable for drug screening and testing potential glaucoma treatments, particularly those targeting trabecular meshwork function.

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For research use only. Not for any other purpose.