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Immortalized Human Oral Keratinocytes

Cat.No.: CSC-I9230L

Species: homo sapiens

Source: Human Oral Mucosa

Culture Properties: Adherent

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Cat.No.
CSC-I9230L
Description
Immortalized Human Oral Keratinocytes-hTERT from Creative Bioarray were developed from human tissues were transduced with a lentiviral expression vector containing the hTERT gene. The cell line was continuously cultured for more than 50 population doublings without showing signs of growth retardation or replicative senescence.
Species
homo sapiens
Source
Human Oral Mucosa
Recommended Medium
SuperCult® Immortalized Human Oral Keratinocyte Medium (Cat No.: CM-I9230L)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Immortalization Method
Human telomerase reverse transcriptase (hTERT) and SV40 large T antigen
Applications
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CSC-7745W Human Oral Keratinocytes (HOK)
CIK-HT013 HT® Lenti-hTERT Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
What are Immortalized Human Oral Keratinocytes-hTERT?

Immortalized Human Oral Keratinocytes-hTERT are oral keratinocyte cells derived from human sources that have been modified with the human telomerase reverse transcriptase (hTERT) gene. This modification allows the cells to go beyond 20 passages while retaining essential characteristics of primary oral keratinocytes.

What research applications are Immortalized Human Oral Keratinocytes-hTERT suitable for?

Immortalized Human Oral Keratinocytes-hTERT are suitable for a wide range of applications, including:
Oral cancer research and carcinogenesis studies.
Oral biology and tissue engineering.
Wound healing and regenerative medicine.
Drug testing and toxicity assays relevant to oral tissues.
Studies on cell differentiation, signaling, and interactions in the oral epithelium.

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For research use only. Not for any other purpose.