Immortalized Human Nasal Epithelial Cells-SV40

Cat.No.: CSC-I9157L

Species: homo sapiens

Source: Nasal Mucosa

Morphology: Polygonal

Culture Properties: Adherent

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Cat.No.

CSC-I9157L

Description

Nasal epithelial cells form the outermost protective layer against environmental factors. They clean, humidify, and warm inhaled air. They produces mucus, which binds particles that are subsequently transported to the pharynx by cilia on the epithelial cells. It has been shown that epithelial cells not only defend physically, but also respond by the production of inflammatory mediators that may affect local immune responses. They are used as an in vitro model for studying nasal defence mechanism.

Species

homo sapiens

Source

Nasal Mucosa

Recommended Medium

SuperCult® Immortalized Human Nasal Epithelial Cell Medium (Cat No.: CM-I9157L)

Freezing Medium

Complete medium supplemented with 10% (v/v) DMSO

Culture Properties

Adherent

Morphology

Polygonal

Immortalization Method

SV40 large T antigen

Markers

Cytokeratin

Applications

For Research Use Only

Growth Properties

Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂.

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20 °C.

Shipping

Dry Ice.

Recommended Products

CSC-C1676 Human Nasal Epithelial Cells (HNEpC)
CIK-HT003 HT® Lenti-SV40T Immortalization Kit

Quality Control

Real Time PCR was used to quantify SV40T gene expression in immortalized cell line. Free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations.

BioSafety Level

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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