Immortalized Human Mammary Epithelial Progenitor (K5+/K19+) Cells-hTERT

Cat.No.: CSC-I9211L

Species: Homo sapiens

Source: Mammary

Morphology: Cobblestone or Spindle-shaped

Culture Properties: Adherent

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Cat.No.

CSC-I9211L

Description

The immortalized human mammary progenitor cells – hTERT (K5+/K19+) is a clonal cell population of progenitors coexpressing normal mammary and stem cell markers. It has the ability to self-renew and differentiate into luminal and/or myoepithelial cell lineages. Through propagation in different optimized media, the cells are able to give rise to new population of cells with different morphology and characteristics, such as mucin-1 positive cells, vimentin-negative cells, or expressing basal, luminal, and other stem cell markers. Aldehyde dehydrogenase 1A3 enzyme is noticeably higher in expression, a marker commonly used for isolating normal and tumor mammary stem cells. K5+/K19+cells have Wnt, Notch, and hedeghog gene regulatory pathways. It is a valuable tool in research in the field of biology, the stem/progenitor origin, and the heterogeneity mechanisms in breast cancer.

Species

Homo sapiens

Source

Mammary

Culture Properties

Adherent

Morphology

Cobblestone or Spindle-shaped

Immortalization Method

Serial passaging and transduction with recombinant lentiviruses carrying hTERT gene

Markers

K5, K14, vimentin, E-cadherin, p63, K8, K18, K19, CD29, CD49fa-SMA, CD10, Thy-1 when grown in DCFI-1

Applications

For Research Use Only

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20 °C.

Shipping

Dry Ice.

Recommended Products

CIK-HT013 HT® Lenti-hTERT Immortalization Kit

Quality Control

1) Western blot to assess cell lineage-related and stem cell markers;
2) Immunofluorescence staining analysis for lineage markers;
3) Gene expression profiling for gene regulatory pathways.

BioSafety Level

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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