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Immortalized Human Mammary Epithelial Cells

Cat.No.: CSC-I9138L

Species: homo sapiens

Source: Mammary Gland

Morphology: Polygonal

Culture Properties: Adherent

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Cat.No.
CSC-I9138L
Description
Immortalized Human Mammary Epithelial Cells were derived from primary human tissue transduced with a lentiviral expression vector containing the SV40T gene. The cell line was continuously cultured for more than 20 passages without showing signs of growth retardation or replicative senescence.
Species
homo sapiens
Source
Mammary Gland
Recommended Medium
SuperCult® Immortalized Human Mammary Epithelial Cell Medium (Cat No.: CM-I9138L)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
SV40 large T antigen
Markers
CD10+, MUC1-, cytokeratin
Applications
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CSC-C4038X Human Mammary Epithelial Cells (HMEpC)
CIK-HT013 HT® Lenti-hTERT Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
Real Time PCR was used to quantify SV40T gene expression in immortalized cell line. Free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.