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Immortalized Human Hepatocytes-SV40

Cat.No.: CSC-I9016L

Species: homo sapiens

Source: Liver

Morphology: Polygonal

Culture Properties: Adherent

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Cat.No.
CSC-I9016L
Description
Immortalized Human Hepatocytes-SV40 from Creative Bioarray were developed from human tissues transduced with a lentiviral expression vector containing the SV40T gene. The cell line was continuously cultured for more than 30 passages without showing signs of growth retardation or replicative senescence.
Species
homo sapiens
Source
Liver
Recommended Medium
SuperCult® Immortalized Human Hepatocytes Plating Medium (Cat No.: CM-I9016L-P)
SuperCult® Immortalized Human Hepatocytes Culture Medium (Cat No.: CM-I9016L-C)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
SV40 large T antigen
Markers
CK18, CK19
Applications
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CSC-C1495 Human Hepatocytes
CIK-HT003 HT? Lenti-SV40T Immortalization Kit
Quality Control
Real Time PCR was used to quantify SV40T gene expression in immortalized cell line.
Free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
What culture medium is recommended for Immortalized Human Hepatocytes-SV40?

For optimal growth and maintenance, Immortalized Human Hepatocytes-SV40 should be cultured in a suitable medium. We recommend using:
SuperCult® Immortalized Human Hepatocytes Plating Medium (Cat No.: CM-I9016L-P)
SuperCult® Immortalized Human Hepatocytes Culture Medium (Cat No.: CM-I9016L-C)

What research applications are Immortalized Human Hepatocytes-SV40 suitable for?

Immortalized Human Hepatocytes-SV40 are widely used in various research applications, including:
Studies on liver metabolism and toxicity.
Drug metabolism and pharmacokinetics.
Research on mechanisms of liver disease and liver cancer.
Investigating liver-specific biomolecule storage and release.

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For research use only. Not for any other purpose.