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Immortalized Human Hepatic Stellate Cells-SV40T

Cat.No.: CSC-I2096Z

Species: homo sapiens

Morphology: Polygonal

Culture Properties: Adherent

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Cat.No.
CSC-I2096Z
Description
Immortalized Human Hepatic Stellate Cells-SV40 were developed from human tissues transduced with a lentiviral expression vector containing the SV40T gene. The cell line was continuously cultured for more than 30 passages without showing signs of growth retardation or replicative senescence.
Species
homo sapiens
Recommended Medium
SuperCult® Immortalized Human Hepatic Stellate Cell Medium (Cat No.: CM-I2096Z)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
SV40 large T antigen and Human telomerase reverse transcriptase (hTERT)
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Shipping
Dry Ice.
Quality Control
Real Time PCR was used to quantify SV40T gene expression in immortalized cell line. Free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations.
Storage and Shipping
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20°C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
Why are hepatic stellate cells important for research?

Hepatic stellate cells are critical for studying liver biology and pathophysiology. They are involved in processes such as liver fibrosis, inflammation, and regeneration. Research on these cells helps in understanding liver diseases, including cirrhosis and non-alcoholic fatty liver disease (NAFLD), and developing therapeutic strategies.

What applications can be pursued with these cells?

Immortalized Human Hepatic Stellate Cells can be utilized in various research applications, including:
Investigating the mechanisms of liver fibrosis and tissue remodeling.
Testing the effects of drugs and compounds on stellate cell activation.
Studying the interactions between stellate cells and other liver cell types (e.g., hepatocytes, Kupffer cells).
Exploring the role of hepatic stellate cells in liver inflammation and regeneration.

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For research use only. Not for any other purpose.