Immortalized Human Glomerular Podocytes-SV40 (Tet-on)

Cat.No.: CSC-I1910Z

Species: homo sapiens

Morphology: Polygonal

Culture Properties: Adherent

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Cat.No.

CSC-I1910Z

Description

Human Glomerular Podocytes were isolated and transfected with SV40LT expressing lentiviral particles under the control of a Tet-on system in the presence of DOX. The glomerular podocyte is believed to play a role in the development and progression of albuminuria and glomer-ulosclerosis associated with diabetes. This cell line may be utilized as an in vitro model.

Species

homo sapiens

Recommended Medium

SuperCult? Immortalized Human Glomerular Podocyte Medium (Cat No.: CM-I1910Z)

Freezing Medium

Complete medium supplemented with 10% (v/v) DMSO

Culture Properties

Adherent

Morphology

Polygonal

Immortalization Method

SV40 large T antigen

Applications

For Research Use Only

Growth Properties

Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂.

Shipping

Dry Ice.

Recommended Products

CIK-HT003 HT? Lenti-SV40T Immortalization Kit
CIK-HT004 HT? Lenti-SV40 (tsA58 temperature sensitive mutant) Lentivirus Immortalization Kit

Quality Control

Real Time PCR was used to quantify SV40T gene expression in immortalized cell line. Free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations.

Storage and Shipping

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20 °C.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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