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Immortalized Human Gingival Keratinocytes (Gie-No3B11)
Cat.No.: CSC-I9236L
Species: homo sapiens
Source: Buccal Gingiva
Morphology: Polygonal
Culture Properties: Adherent
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Cat.No.
CSC-I9236L
Description
Immortalized Human Gingival Keratinocytes-hTERT were developed from human tissues transduced with a lentiviral expression vector containing the hTERT gene. The cell line was continuously cultured for more than 20 passages without showing signs of growth retardation or replicative senescence.
Species
homo sapiens
Source
Buccal Gingiva
Recommended Medium
SuperCult® Immortalized Human Gingival Keratinocyte Medium (Cat No.: CM-I9236L)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
Human telomerase reverse transcriptase (hTERT)
Applications
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CIK-HT013 HT® Lenti-hTERT Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
Real Time PCR was used to quantify hTERT gene expression in immortalized cell line.
Free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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