Description
Immortalized Human Fallopian Tube Secretory Epithelial Cells-hTERT were developed from human tissues transduced with a lentiviral expression vector containing the hTERT gene. The cell line was continuously cultured for more than 20 passages without showing signs of growth retardation or replicative senescence.
Source
Fimbriae of fallopian tube
Recommended Medium
SuperCult® Immortalized Human Fallopian Tube Secretory Epithelial Cell Medium (Cat No.: CM-I9204L)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Morphology
Cobblestone-like
Immortalization Method
Human telomerase reverse transcriptase (hTERT)
Applications
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Recommended Products
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
CIK-HT013 HT® Lenti-hTERT Immortalization Kit
Quality Control
Real Time PCR was used to quantify hTERT gene expression in immortalized cell line.
Free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations.
Citation Guidance
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Can these cells be frozen and thawed for storage?
Yes, Immortalized Human Fallopian Tube Secretory Epithelial Cells can be cryopreserved for long-term storage. Following established freezing protocols helps maintain cell viability upon thawing. It's essential to carefully follow guidelines for freezing and thawing to preserve cell health.
Can these cells be used for drug testing?
Yes, these cells can be utilized for drug testing and toxicity studies related to reproductive health. Their immortalized nature and similarity to human tissue make them an excellent choice for screening potential therapeutic compounds.