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Immortalized Human Dermal Microvascular Endothelial Cells
Cat.No.: CSC-I9095L
Species: homo sapiens
Source: Skin
Morphology: Polygonal
Culture Properties: Adherent
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Cat.No.
CSC-I9095L
Description
Immortalized Human Dermal Microvascular Endothelial Cells provided by Creative Bioarray have been developed by immortalizing primary human dermal endothelial cells with SV40 Large T antigen. Immortalized cells were controlled passaging side by side with the primary cells. Primary cells go into senescence after the 3rd passage while the SV40‐tranduced cells go beyond 30 passages.
Species
homo sapiens
Source
Skin
Recommended Medium
SuperCult® Immortalized Human Dermal Microvascular Endothelial Cell Medium (Cat No.: CM-I9095L)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
SV40 large T antigen
Markers
Factor VIII- related antigen
Applications
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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CIK-HT013 HT® Lenti-hTERT Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
CIK-HT013 HT® Lenti-hTERT Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
Real Time PCR was used to quantify SV40T gene expression in immortalized cell line.
free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations
free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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