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Immortalized Human Carotid Artery Smooth Muscle Cells-SV40
Cat.No.: CSC-I9070L
Species: homo sapiens
Source: Heart
Morphology: Multipolar
Culture Properties: Adherent
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Cat.No.
CSC-I9070L
Description
Immortalized Human Carotid Artery Smooth Muscle Cells-SV40 provided by Creative Bioarray have been developed by immortalizing primary human brain endothelial cells with SV40. The cell line was continuously cultured for more than 20 passages without showing signs of growth retardation or replicative senescence.
Species
homo sapiens
Source
Heart
Recommended Medium
SuperCult® Immortalized Human Carotid Artery Smooth Muscle Cell Medium (Cat No.: CM-I9070L)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Morphology
Multipolar
Immortalization Method
SV40 large T antigen
Markers
alpha-Actin
Applications
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CSC-C9334W Human Carotid Artery Smooth Muscle Cells
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
Real Time PCR was used to quantify SV40T gene expression in immortalized cell line.
Free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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