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Immortalized Human Bronchial Epithelial Cells-hTERT+CDK4

Cat.No.: CSC-I9214L

Species: homo sapiens

Source: Lung

Morphology: Polygonal

Culture Properties: Adherent

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Cat.No.
CSC-I9214L
Description
Immortalized Human Bronchial Epithelial Cells-hTERT+CDK4 provided by Creative Bioarray have been developed by immortalizing primary Human Bronchial Epithelial Cells with hTERT and CDK4 gene. The cell line was continuously cultured for more than 20 passages without showing signs of growth retardation or replicative senescence.
Species
homo sapiens
Source
Lung
Recommended Medium
SuperCult® Immortalized Human Bronchial Epithelial Cell Medium (Cat No.: CM-I9214L)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
Human telomerase reverse transcriptase (hTERT) CDK4
Markers
Cytokeratin
Applications
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CSC-C4872L Human Bronchial Epithelial Cells
CIK-HT013 HT® Lenti-hTERT Immortalization Kit
Quality Control
Real Time PCR was used to quantify hTERT gene and CDK4 expression in immortalized cell line. Free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.