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Immortalized Human Ascending Aorta Vascular Smooth Muscle Cells

Cat.No.: CSC-I9328L

Species: homo sapiens

Source: Heart

Morphology: Polygonal (varies depending on phenotype)

Culture Properties: Adherent

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Cat.No.
CSC-I9328L
Description
Vascular Smooth Muscle Cells (VSMCs) can perform contractile as well as synthetic functions which are associated with changes in morphology, proliferation rates, and the expression of different marker proteins such as TGFβ. Immortalized Human Ascending Aorta Vascular Smooth Muscle Cells are isolated from a male patient with bicuspid aortic valve (BAV). The phenotype varies from quiescent and contractile to proliferative and synthetic which can perform an array of functions depending on the phenotype. The immortalized cells are valuable for research studies of the expression and regulation of genes particularlyendoglin. Endoglinis downregulated in the Immortalized Human Ascending Aorta Vascular Smooth Muscle Cells in response to treatment with the drug Losartan (Cozaar) in various concentrations.
Species
homo sapiens
Source
Heart
Recommended Medium
SuperCult® Immortalized Human Ascending Aorta Vascular Smooth Muscle Cell Medium (Cat No.: CM-I9328L)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Morphology
Polygonal (varies depending on phenotype)
Immortalization Method
Human telomerase reverse transcriptase (hTERT)
Applications
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CIK-HT013 HT® Lenti-hTERT Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
Real Time PCR was used to quantify hTERT gene expression in immortalized cell line. Free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
What applications can Immortalized Human Ascending Aorta Vascular Smooth Muscle Cells be used for?

Our Immortalized Human Ascending Aorta Vascular Smooth Muscle Cells can be utilized in a variety of applications, including:
Drug screening and testing for cardiovascular therapies.
Studies on smooth muscle cell differentiation and function.
Investigating the effects of mechanical stress on vascular cells.
Assessing the role of inflammation in vascular diseases.

Are these cells suitable for high-throughput screening?

Yes! Our Immortalized Human Ascending Aorta Vascular Smooth Muscle Cells are well-suited for high-throughput screening assays. Their stable growth and reproducibility make them an ideal choice for evaluating large libraries of drug candidates.

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For research use only. Not for any other purpose.