Immortalized Human Alveolar Cells

Cat.No.: CSC-I9125L

Species: homo sapiens

Source: Lung

Morphology: Polygonal

Culture Properties: Adherent

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Cat.No.

CSC-I9125L

Description

Type I alveolar epithelial cells are large squamous cells, which extend across several air sacs and cover more than 95% of the alveolar surface area. They are specialized to serve as very thin (often only 25 nm in width) gas-permeable components of the blood-air barrier. The Type I Alveolar cells are the target of many insults, including ventilator-induced lung injury. These cells are useful in investigating the function and pathology of the respiratory system. Immortalized Human Alveolar Cells-hTERT were developed from human tissues transduced with a lentiviral expression vector containing the hTERT gene. The cell line was continuously cultured for more than 20 passages without showing signs of growth retardation or replicative senescence.

Species

homo sapiens

Source

Lung

Recommended Medium

SuperCult® Immortalized Human Alveolar Cell Medium (Cat No.: CM-I9125L)

Freezing Medium

Complete medium supplemented with 10% (v/v) DMSO

Culture Properties

Adherent

Morphology

Polygonal

Immortalization Method

Human telomerase reverse transcriptase (hTERT) and SV40 large T antigen

Markers

CK-18, CK-19

Applications

For Research Use Only

Growth Properties

Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂.

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20 °C.

Shipping

Dry Ice.

Recommended Products

CSC-C9223J Human Alveolar Epithelial Cells
CIK-HT013 HT® Lenti-hTERT Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit

BioSafety Level

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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