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Immortalized Human Adipose Tissue-Derived Mesenchymal Stem Cells-SV40 (Tet-on)

Cat.No.: CSC-I1908Z

Species: homo sapiens

Morphology: Polygonal

Culture Properties: Adherent

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Cat.No.
CSC-I1908Z
Description
Human dipose tissue-derived mesenchymal stem cells were isolated from human adipose tissues and transfected with SV40LT expressing lentiviral particles under the control of a Tet-on system in the presence of DOX. This cell line may be utilized as an in vitro model for clinical and experimental use due to their capacity to self-renew and differentiate into multiple mesenchymal lineages.
Species
homo sapiens
Recommended Medium
SuperCult? Immortalized Human Adipose Tissue-Derived Mesenchymal Stem Cell Medium (Cat No.: CM-I1908Z)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
SV40 large T antigen
Applications
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Shipping
Dry Ice.
Recommended Products
CIK-HT003 HT? Lenti-SV40T Immortalization Kit
CIK-HT004 HT? Lenti-SV40 (tsA58 temperature sensitive mutant) Lentivirus Immortalization Kit
Quality Control
Real Time PCR was used to quantify SV40T gene expression in immortalized cell line. Free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations.
Storage and Shipping
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.