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Immortalized Canine Skeletal Muscle Cells

Cat.No.: CSC-I9359Z

Species: Canine

Morphology: Polygonal

Culture Properties: Adherent

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Cat.No.
CSC-I9359Z
Description
Immortalized Canine Skeletal Muscle Cells-SV40 were derived from primary canine tissue transduced with a lentiviral expression vector containing the SV40T gene. The cell line was continuously cultured for more than 20 passages without showing signs of growth retardation or replicative senescence.
Species
Canine
Recommended Medium
SuperCult® Immortalized Canine Skeletal Muscle Cell Medium (Cat No.: CM-I9359Z)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
SV40 large T antigen
Applications
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Shipping
Dry Ice.
Quality Control
Real Time PCR was used to quantify SV40T gene expression in immortalized cell line. Free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations.
Storage and Shipping
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20 °C.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.