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Immortalize Human Cervical Epithelial Cells
Cat.No.: CSC-I2112Z
Species: homo sapiens
Morphology: Polygonal
Culture Properties: Adherent
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Cat.No.
CSC-I2112Z
Description
Immortalize Human Cervical Epithelial Cells were developed from human tissues were immortalized by transduction with the retroviral vector LXSN-16E6E7 in the presence of polybrene. The cell line was continuously cultured for more than 30 passages without showing signs of growth retardation or replicative senescence.
Species
homo sapiens
Recommended Medium
SuperCult® Immortalize Human Cervical Epithelial Cell Medium (Cat No.: CM-I2112Z)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
HPV16 E6/E7 gene
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Shipping
Dry Ice.
Quality Control
Real Time PCR was used to quantify HPV16 E6/E7 gene expression in immortalized cell line.
free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations
free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations
Storage and Shipping
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20°C.
Note: Never can cells be kept at -20°C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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