Description
SK-GT-4 was established from a primary tumour in 1989 from a 89 year-old Caucasian male who presented with dysphagia secondary to a well-differentiated adenocarcinoma arising in the Barrett epithelium of the distal oesophagus. The tumour invaded into but not through the muscle layer (T2) and involved 3 of 14 lymph nodes (N1). SK-GT-4 was found to be tumorigenic in athymic nu/nu mice.
Tissue of Origin
Distal Oesophagus
Recommended Medium
RPMI-1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)
Storage
Liquid Nitrogen (-180 °C).
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Never can cryopreserved cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
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What are the methods to test the growth-promoting effect of serum?
Clone formation rate, vialing rate measurement method and continuous passaging culture method.