KYSE-180

Cat.No.: CSC-C0427

Species: Human

Source: esophageal squamous cell carcinoma

Morphology: epitheloid cells growing as adherent monolayer

Culture Properties: monolayer

Inquire for Price

Specification
Q & A
Customer Review

Cat.No.

CSC-C0427

Description

Established from the well differentiated esophageal squamous cell carcinoma resected from middle intra-thoracic esophagus of a 53-year-old Japanese man prior to treatment (tumor was invading contiguous structures)

Species

Human

Source

esophageal squamous cell carcinoma

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

monolayer

Morphology

epitheloid cells growing as adherent monolayer

Karyotype

Human hyperdiploid karyotype with 11% polyploidy - 55(50-57)<2n>XX, +1, +1, +3, +7, +7, +8, -14, +16, -17, -17, -18, -18, +5-8mar, del(X)(q23), add(1)(q32), der(1;6)(q10;p10), der(1;13)(q10;q10), add(3)(p26), add(5)(q25), add(6)(p11), del(6)(q23), add(7)(

Quality Control

Mycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cy

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is the concentration of serum to be used?

Serum is used as an additive to the synthetic medium, usually at a concentration of 5-20%, most often at 10%.

Ask a Question

Average Rating: 5.0 | 1 Scientist has reviewed this product

Thankful

I am extremely thankful to the staff in the Creative Bioarray for their help.

22 June 2022

Ease of use
After sales services
Value for money

Write your own review

You May Also Need

CM-1448Y

SuperCult® RPMI 1640 Medium

INQUIRY

CS-001Y

PremSera® FBS

INQUIRY

KYSE-140

Description: Derived from the moderately differentiated invasive esophageal squamous cell carcinoma resected from middle intra-thoracic esophagus of a 54-year-old Japanese man prior to treatment (depth of invasion was a1); described to carry p53 mutation

Cat#: CSC-C0404 INQUIRY

View Product

KYSE-30

Description: Derived from well differentiated invasive esophageal squamous cell carcinoma resected from middle intra-thoracic esophagus of a 64-year-old Japanese man prior to treatment; cell line established from tumor cells heterotransplanted into athymic mice; described to be heterotransplantable in athymic mice and to carry p53 mutation and amplification of cERB B, MYC and CYCLIN D1

Cat#: CSC-C0405 INQUIRY

View Product

KYSE-70

Description: Established from the poorly differentiated invasive esophageal squamous cell carcinoma resected from middle intra-thoracic esophagus of a 77-year-old Japanese man prior to treatment (depth of invasion was not beyond the muscularis propria)

Cat#: CSC-C0414 INQUIRY

View Product

KYSE-520

Description: Established from the moderately differentiated invasive eosphageal squamous cell carcinoma resected from lower intra-thoracic esophagus of a 58-year-old Japanese woman prior to treatment (tumor had invaded the adventitia); described as carrying p53 mutation and amplification of the oncogene MYC

Cat#: CSC-C0419 INQUIRY

View Product

KYSE-150

Description: Established from the poorly differentiated esophageal squamous cell carcinoma resected from upper (cervical) esophagus of a 49-year-old Japanese woman after receiving radiotherapy (tumor was invading contiguous structures); described as carrying amplified oncogenes, c-erb-B (8x) and cyclin D1 (4x) and producing tumors in nude mice

Cat#: CSC-C0423 INQUIRY

View Product

KYSE-270

Description: Derived from well differentiated invasive esophageal squamous cell carcinoma resected from middle intra-thoracic esophagus of a 79-year-old Japanese man prior to treatment (depth of invasion was not beyond the muscularis propria); described to carry p53 mutation

Cat#: CSC-C0428 INQUIRY

View Product

For research use only. Not for any other purpose.