KYSE-70

Cat.No.: CSC-C0414

Species: Human

Source: esophageal squamous cell carcinoma

Morphology: epitheloid cells growing adherently in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0414

Description

Established from the poorly differentiated invasive esophageal squamous cell carcinoma resected from middle intra-thoracic esophagus of a 77-year-old Japanese man prior to treatment (depth of invasion was not beyond the muscularis propria)

Species

Human

Source

esophageal squamous cell carcinoma

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

monolayer

Morphology

epitheloid cells growing adherently in monolayers

Karyotype

Human hypotriploid karyotype with 8% polyploidy - 60(54-63)<3n>XXX, -Y, -2, -3, -3, -4, -6, +7, -8, -9, -10, -13, -14, -15, -16, -16, -16, -17, -18, -18, -21, -22, +10mar, del(X)(q25), add(1)(p11), add(2)(q33), del(2)(q33), del(3)(q13), add(4)(q35), add(5

Quality Control

Mycoplasma: contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytoker

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2-4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What treatment should be done before using the serum?

Most cell culture sera do not need to be inactivated prior to use. The purpose of inactivation is to remove the complement component of the serum and to avoid the impact of complement on subsequent experiments.

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Average Rating: 4.0 | 1 Scientist has reviewed this product

Satisfied

Generally speaking, we are very satisfied with Creative Bioarray's products and services.

23 Jan 2021

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