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ActoFactor™ Human basic Fibroblast growth factor 2
- Specification
Cat.No.
CSC-CTK0012G
Description
FGF-2, also known as bFGF, reliable improves proliferation of mesenchymal stem cells (MSCs). It in addition supports proliferation and differentiation of chondrocytes and supports propagation of undifferentiated pluripotent stem cells (PSCs).
Source
E coli.
Sequence
MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS
Molecular Mass
Approximately 16.5 kDa, a single non-glycosylated polypeptide chain containing 147 amino acids
Size
5μg, 100μg, 500μg, 1000μg
Purity
> 98 %
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 ×107 IU/mg
Endotoxin Level
<0.01EU/μg
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of less than 0.3 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Usage
This GMP product can be for research use or further manufacturing use.(NOT FOR HUMAN USE)
Storage
Store lyophilized cytokine at -20°C to -80°C.
Avoid repeated freeze/thaw cycles.
Avoid repeated freeze/thaw cycles.
Shipping
Dry ice
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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