YAPC

Cat.No.: CSC-C0430

Species: Human

Source: pancreas carcinoma

Morphology: cobblestone-like adherent cells growing in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0430

Description

Established from ascites obtained during exploratory laparatomy of a 43-year-old Japanese man with pancreas carcinoma (terminal, no therapy) in December 1993; cells were described as being dependent on autocrinely secreted IL-1alpha

Species

Human

Source

pancreas carcinoma

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

monolayer

Morphology

cobblestone-like adherent cells growing in monolayers

Karyotype

Human near-triploid karyotype with 8% polyploidy - 66-71<3n>XXYY, +3, +7, +8, +9, +10, +14, -17, -18, -19, -20, -22, -22, -22, +3mar, der(1)del(1)(q23-25)inv(1)(p11q21), der(1;5)(q10;q10), add(3)(p11)/del(3)(p11p21)x2, del(5)(p11), add(6)(p21), del(7)(q32

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 (+), cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 3.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

How are adherent cells detached from the culture surface?

To detach adherent cells from the culture surface, various methods can be used. These include enzymatic methods using enzymes like trypsin or collagenase, physical methods like scraping or shaking or using commercially available cell dissociation reagents.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

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Creative Bioarray offers flexible payment options that can meet the needs of researchers with varying budgetary constraints.

25 Dec 2022

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