TT2609-C02

Cat.No.: CSC-C0519

Species: Human

Source: follicular thyroid carcinoma

Morphology: small cells growing adherent but irregularly in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0519

Description

Established in 1996 from the tumor tissue of a 57-year-old Caucasian man with local recurrence of a previously iodine-irradiated follicular thyroid cancer (well-differentiated with dispersed poorly differentiated areas)

Species

Human

Source

follicular thyroid carcinoma

Recommended Medium

80-85% RPMI-1640 + 2 mM L-glutamine + 1 mM sodium pyruvate+ 15-20% h.i.FBS + insulin-transferrin-selentite medium supplement

Culture Properties

monolayer

Morphology

small cells growing adherent but irregularly in monolayers

Karyotype

Human flat-moded hypertriploid karyotype with 4% polyploidy - 69-75<3n>XXYY, -2, +8, +8, +9, +10, +14, -15, -16, +17, -22, +1-2mar, der(1)t(1;2)(p32;q11-13), add(4)(p15.2), ?inv(8)(q12q21.2)x1-2, add(9)(p24)x2, i(15q), del(17)(p11)x1-2, add(22)(p11)x2 - r

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, thyroglobulin -, vimentin -
Viruses: PCR:

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What are some common assays used to study the functional characteristics of thyroid tumor cells?

Several assays are used to study the functional characteristics of thyroid tumor cells. These include cell viability assays (such as MTT or XTT assays), proliferation assays (such as BrdU or EdU incorporation), apoptosis assays (such as Annexin V staining), migration and invasion assays (such as Transwell or scratch assays), and clonogenic assays.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Easy to culture

I'm impressed with the performance of this cell products. The cells were easy to culture, and their phenotype remained stable throughout my experiments.

09 Oct 2023

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For research use only. Not for any other purpose.