TC-71

Cat.No.: CSC-C0525

Species: Human

Source: Ewing's sarcoma

Morphology: loosely adherent cells growing in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0525

Description

Established from the tumor of a 22-year-old man with metastatic Ewings sarcoma that arose in the humerus; cell line was derived in 1981 from a biopsy of recurrent tumor at the primary site

Species

Human

Source

Ewing's sarcoma

Recommended Medium

90% RPMI-1640 with 2mM L-glutamine + 10% h.i. FBS

Culture Properties

monolayer

Morphology

loosely adherent cells growing in monolayers

Karyotype

Human flat-moded hypertriploid karyotype with 4% polyploidy - 70-80<3n>XX, -Y, +1, +2, -3, +6, +8, -9, +11, -17, +21, +2-11 mar, add(3)(q29), add(6)(q25)x2-3, del(8)(p22), add(8)(p22), del(9(p24), t(11;22)(q24;q12)x2, i(13q)x1-2, add(14)(p11) - sideline w

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, cytokeratin-19 -, desmin -, endothel -, EpCAM -, GFAP -, neurofilament +, vimentin +
Viruses: PCR:

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

How do scientists ensure the accuracy and reproducibility of cell viability assay results?

Scientists follow strict protocols, perform multiple replicates, control experimental conditions, and use appropriate positive and negative controls to ensure accurate and reproducible results.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Excellent services

I am grateful for the excellent service attitude displayed by Creative Bioarray. They were prompt in addressing our queries, provided technical assistance when needed, and ensured a seamless experience from ordering to delivery.

05 Feb 2023

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For research use only. Not for any other purpose.