CAL-72

Cat.No.: CSC-C0473

Species: Human

Source: osteosarcoma

Morphology: fibroblastoid cells growing adherently in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0473

Description

Established from a chemotherapy-resistant tumor sample obtained from the left knee of a 10-year-old boy with anaplastic osteoblastic sarcoma (Rosen grade IIB) in 1989

Species

Human

Source

osteosarcoma

Recommended Medium

90% DMEM + 10% h.i.FBS + 1x insulin-transferrin-sodium selenite

Culture Properties

monolayer

Morphology

fibroblastoid cells growing adherently in monolayers

Karyotype

Human, bimodal hyperdiploid/hypertetraploid karyotype with 12% polyploidy - 45-56<2n>X, -Y, +1, +1, -2, -2, +8, +11, -13, -15, +19, -20, -20, -22, -22, +15-20mar, +dmin, add(X)(q21), der(1;19)(q10;q10), add(1)(p3?5)add(1)(q3?), add(3)(q2?9)x2, add(6)(q11)

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin -, desmin -, endothel -, GFAP -, HMB-45 -, neurofilament -, vimentin +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Can cell viability assays distinguish between apoptotic and necrotic cell death?

Some assays, such as Annexin V staining or caspase activity assays, can differentiate between apoptotic and necrotic cell death by measuring specific markers or enzymatic activities.

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Average Rating: 4.0 | 1 Scientist has reviewed this product

Broad applications

The scientific application of Creative Bioarray's retinoblastoma cells has broadened the scope of our research.

27 Feb 2023

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For research use only. Not for any other purpose.