RH-30

Cat.No.: CSC-C0500

Species: Human

Source: rhabdomyosarcoma

Morphology: adherent epithelial-like cells growing as monolayer

Culture Properties: monolayer

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Cat.No.

CSC-C0500

Description

Established from the bone marrow metastasis of a 17-year-old man with undifferentiated (standard classification) or unclassified (Palmer classification) alveolar rhabdomyosarcoma; cells were described as carrying a p53 mutation, expressing high levels of both myogenin and myoD, and expressing the Pax3/FKHR fusion protein secondary to the t(2;13)(q35;q14) translocation

Species

Human

Source

rhabdomyosarcoma

Recommended Medium

90-95% RPMI-1640 + 5-10% h.i. FBS

Culture Properties

monolayer

Morphology

adherent epithelial-like cells growing as monolayer

Karyotype

Human flat moded hypotetraploid karyotype with 20% polyploidy - 78-87<4n>XX, -3, -4, -4, +5, +7, +7, -10, -10, -12, -12, -15, -19, +20, +20, +20, -21, +1-2mar, +0-4 dmin, add(1)(p11), del(5)(q13), add(16)(p13), del(16)(p13.2) - sideline with i(3q)

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin -, desmin +, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

How is a Trypan blue exclusion assay used to determine cell viability?

The Trypan blue exclusion assay involves staining dead cells with Trypan blue, which cannot penetrate the membrane of live cells, allowing for differentiation between live and dead cells under a microscope.

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Average Rating: 4.0 | 1 Scientist has reviewed this product

Reliable products

The retinoblastoma cells we purchased from Creative Bioarray have been an integral part of our research project. Their consistent and reliable behavior has allowed us to generate robust and reproducible data.

07 May 2023

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For research use only. Not for any other purpose.