M3E3/C3

Cat.No.: CSC-C2758

Species: Hamster

Source: fetal lung

Morphology: epithelial-like cells growing in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C2758

Description

Established from the lung of a Syrian golden hamster fetus on day 15 after gestation in 1981; primary culture cells were treated with hydroxy-proline; cells were described as premature epithelial cells with the ability to differentiate into mucus-producing pulmonary cells

Species

Hamster

Source

fetal lung

Recommended Medium

90%RPMI-1640 + 2 mM L-glutamine + 1 mM sodium pyruvate + 10% h.i. FBS

Culture Properties

monolayer

Morphology

epithelial-like cells growing in monolayers

Karyotype

Near-diploid Syrian hamster karyotype with 10% polyploidy - 43-47<2n>

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.