CHO-DHFR-

Cat.No.: CSC-C6243X

Species: Hamster

Source: ovary cells

Morphology: epithelial-like adherent monolayer

Culture Properties: monolayer

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Cat.No.

CSC-C6243X

Description

Subclone of the parental line CHO which was derived from the ovary of an adult Chinese hamster in 1957; the cells are dehydrofolate reductase negative (dhfr-; Dukx cells); patented cell line

Species

Hamster

Source

ovary cells

Recommended Medium

90% SuperCult® AlphaMEM without Nucleosides without L-Glutamine + 10% h.i. FBS + 2 mM L-glutamine (in case other media, e.g. DMEM or IMDM, are used, hypoxanthine and thymidine should be added as supplements)

Culture Properties

monolayer

Morphology

epithelial-like adherent monolayer

Karyotype

Near diploid hamster karyotype with 20% polyploidy - 21(20-22)<2n> - distinctive CHO morphology

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, PCR assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.