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KB-V1

Cat.No.: CSC-C6212X

Species: Human

Source: cervix carcinoma (derivative of HELA)

Morphology: epitheloid-like, sometimes round cells growing in loosely adherent monolayers when vinblastine is present

Culture Properties: monolayer

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Cat.No.
CSC-C6212X
Description
KB-V1 is a multidrug-resistant (mdr) subclone derived in 1985 from KB-3-1 which itself is a derivative of the 1951 established HELA (31-year-old black woman); cells express strongly mdr1 mRNA and protein; the cell line will only be distributed to non-profit institutions, commercial entities may apply for a license at the NIH/NCI Technology Transfer Cernter, Rockville, MD, USA, phone (301)435-3111
Species
Human
Source
cervix carcinoma (derivative of HELA)
Recommended Medium
85% DMEM + 15% h.i.FBS + 200-1000 ng/ml vinblastine
Culture Properties
monolayer
Morphology
epitheloid-like, sometimes round cells growing in loosely adherent monolayers when vinblastine is present
Karyotype
Human flat-moded hypotriploid karyotype with 13% polyploidy - 71-77<3n>XX, -X, +1, +3, +5, +5, +5, +7, +7, +8, +8, -9, -11, +12, +15, -16, +17, -18, -19, +21, -22, +mar, der(1;3)(q10;p10), del(3)(q13), der(3;5)(p10;q10), i(5p)x3, del(7)(q10), der(7)add(7)
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, H
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
What are the current research directions in understanding lymph node metastasis?

Current research focuses on identifying molecular mechanisms of lymph node metastasis, developing imaging techniques for early detection, and exploring targeted therapies to prevent metastatic spread.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

Exceptional performance

The cell biology products demonstrated exceptional performance, allowing for precise and accurate cell culture maintenance and experimentation.

02 Oct 2023


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For research use only. Not for any other purpose.