KB-3-1

Cat.No.: CSC-C6211X

Species: Human

Source: cervix carcinoma (derivative of HELA)

Morphology: epithelial-like, adherent cell line growing in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C6211X

Description

The parental continuous cell line HELA was established from the primary cervix carinoma of a 31-year-old black woman 1951; KB-3-1 is a derivative of HELA and is used in multiple drug resistance studies giving raise to drug-resistant mutants (e.g. KB-V1 resistant to vinblastine; KB-CHR-8-5 resistant to colchicine); cells express MYC mRNA; the cell line will only be distributed to non-profit institutions, commercial entities may apply for a license at the NIH/NCI Technology Transfer Cernter, Rockville, MD, USA, phone (301)435-3111

Species

Human

Source

cervix carcinoma (derivative of HELA)

Recommended Medium

90% DMEM + 10% h.i.FBS + 1 mM sodium pyruvate

Culture Properties

monolayer

Morphology

epithelial-like, adherent cell line growing in monolayers

Karyotype

Human hypertriploid karyotype with 1.8% polyploidy - 76(73-78)<3n>XX, -X, -1, -3, -4, +6, +7, +8, -9, -18, -20, +11mar, der(1)t(1;?)((p11;?), der(3)t(3;?)(p11;?), der(3) t(3;?)(p14;?), der(3p;5q), del(6)(q21), del(7)(q31), der(8)t(8;?11) (q24;q11), der(9)

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
Viruses: ELISA

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Are there any specific biomarkers associated with lymph node metastasis?

Biomarkers such as podoplanin, D2-40, and certain microRNAs have been studied for their potential role in predicting lymph node metastasis.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Easy to culture

The cells were easy to culture, and their phenotype remained stable throughout my experiments.

11 Oct 2023

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For research use only. Not for any other purpose.