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HIT-T15
Cat.No.: CSC-C9429L
Species: Hamster
Source: pancreas; islet of Langerhans; beta cell
Morphology: epithelial
Culture Properties: monolayer
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Production: insulin
Note: This line was established from a primary culture of Syrian hamster islet cells which were transformed with SV40. The cells secrete up to 24ng of insulin in per 10 exp6 cells per 24 hours(passage 60). Insulin in secretion is stimulated by glucose and glucagon, and is suppressed by somatostatin and glucocorticoids. Insulin synthesis decreases with length of time in culture.
Shipping Condition: Room Temperature
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Description: Established from diethylstilbesterol-induced renal tumors of a 14-month-old male Syrian Golden Hamster in 1997; cells were described as expressing estrogen receptors, glial fibrillary acidic protein and other markers of the neuroectodermal lineage and as being responsive to estrogen
Description: Established after spontaneous transformation of cells isolated from the lung of a normal Chinese hamster (male). The G1 phase in these cells is either absent or very short. Consequently they have been used in studies of the G1 growth period. V79 is well established in toxicology studies. Stability of karyotype and morphology is making them suitable for gene toxicity assays with low background aberrations. The cells do not express endogenous cytochrome P450 and have been used for the insertion and expression of P450 from human, rat, mouse and fish. The cells have a high plating efficiency (80%), and a generation time of 12 to 14 hours. The line was renamed V79 by Elkind in 1958.
Description: Established from the lung of a Syrian golden hamster fetus on day 15 after gestation in 1981; primary culture cells were treated with hydroxy-proline; cells were described as premature epithelial cells with the ability to differentiate into mucus-producing pulmonary cells
