H-2171

Cat.No.: CSC-C0552

Species: Human

Source: small cell lung carcinoma

Morphology: large round cells growing singly or in clumps in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0552

Description

Derived from the pleural effusion of a 50-year-old white male with extensive small cell lung carcinoma after chemotherapy in 1989; corresponds to NCI-H2171

Species

Human

Source

small cell lung carcinoma

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS

Culture Properties

suspension

Morphology

large round cells growing singly or in clumps in suspension

Karyotype

Human hypodiploid karyotype with 8% polyploidy - 42-44<2n>XY, -4, -5, -8, -10, -11, -13, -15, -17, -19, +20, +4-5mar, add(1)(p36), der(2)ins(2;?)(p2?;?)t(2;2)(q37;p24), add(3)(p11), i(3q), del(6)(q25q26), del(9)(p11), der(12)add(12)(p11)del(12)(q24), add(

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 -, desmin -, endothel -, GFAP -, neurofilament -, vimentin -
Viruses: PCR: EBV -, HBV -, HCV -, HIV

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 10 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is the most common cell viability assay?

Tetrazolium reduction assays, ATP cell viability assays, resazurin reduction cell viability assays, live-cell protease viability assays, real-time cell viability assays.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Excellent quality

I am extremely satisfied with the tumor cells I purchased from Creative Bioarray. The cells were of excellent quality and performed well in my experiments.

10 Mar 2023

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For research use only. Not for any other purpose.