BEN

Cat.No.: CSC-C0354

Species: Human

Source: lung carcinoma

Morphology: adherent, epitheloid cells growing in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0354

Description

Established from the supraclavicular tumor cells-containing lymph node of a 71-year-old man with poorly differentiated carcinoma of the lung

Species

Human

Source

lung carcinoma

Recommended Medium

90% DMEM + 10% h.i.FBS

Culture Properties

monolayer

Morphology

adherent, epitheloid cells growing in monolayers

Karyotype

Human highly rearranged flat-moded near-tetraploid karyotype - 82-92<4n>X/XY, -X, -Y, -Y, -2, -2, -3, -3, -4, -4, -5, +6, +6, +7, -8, -8, -9, -10, -13, -13, -14, -16, -16, -17, -18, -18, -19, -21, -21, -21, -22, +18mar - del(1)(q22)x2, add(1)(p11), i(2q),

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vime

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2-3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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