CAL-54

Cat.No.: CSC-C2548

Species: Human

Source: kidney carcinoma

Morphology: epitheloid, adherent cells growing in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C2548

Description

Established from the malignant pleural effusion of a 75-year-old man with kidney carcinoma in 1987

Species

Human

Source

kidney carcinoma

Recommended Medium

85% DMEM +15% h.i.FBS + 0.4 μg/ml hydrocortisone + 10 ng/ml EGF

Culture Properties

monolayer

Morphology

epitheloid, adherent cells growing in monolayers

Karyotype

Human hyperdiploid karyotype with 12% polyploidy - 52(52-53)<2n>X, -Y, +2, +3, +7, +7, +8, +12, -13, +15, +16, +17, del(3)(p21), del(5)(p14.2p15.2), dup(7)(q11q22), add(14)(p12) - del(3p), gain of ch 7 and loss of Y typical of renal cell carcinoma

Quality Control

Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What cells may develop into kidney cancer?

Renal cell carcinoma usually develops as a single tumor in one kidney, but it can affect both kidneys. The cancer begins in the cells that line your kidney's tubules (tiny tubes that return nutrients and fluid back to your blood). The most common type of RCC is clear cell renal cell carcinoma (ccRCC).

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Average Rating: 4.0 | 1 Scientist has reviewed this product

Wonderful brand

Creative Bioarray is a good brand in the industry, and we have maintained good relationship yearly.

02 Mar 2022

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For research use only. Not for any other purpose.