BFTC-909

Cat.No.: CSC-C2549

Species: Human

Source: kidney transitional cell carcinoma

Morphology: epithelial-like (spindle) cells growing in monolayers; cells contain considerable amounts of small, dark granules

Culture Properties: monolayer

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Cat.No.

CSC-C2549

Description

Established from the sarcomatoid component of a grade III transitional cell carcinoma of the renal pelvis of a 64-year-old man in 1990; patient lived in a blackfoot disease endemic area in Taiwan

Species

Human

Source

kidney transitional cell carcinoma

Recommended Medium

80-90% DMEM + 10-20% 10% h.i. FBS.

Culture Properties

monolayer

Morphology

epithelial-like (spindle) cells growing in monolayers; cells contain considerable amounts of small, dark granules

Karyotype

Human flat-moded hypertriplod karyotype with 32% polyploidy - 66-79<3n>XX, -Y, +1, +3, +5, +7, -9, -11, +12, +17, -18, +19, +20, +21, +mar, add(1)(p34)x2, del(2)(p16), +dic(2;9)(q25;p22)x2, add(3)(p24)x2, add(6)(p23)x2, i(7p)x2, i(8q)x2, del(11)(q?22q23),

Quality Control

Mycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What happens to the cells in kidney cancer?

Kidney cancer begins when healthy cells in 1 or both kidneys change and grow out of control, forming a mass called a renal cortical tumor. A tumor can be malignant, indolent, or benign. A malignant tumor is cancerous, meaning it can grow and spread to other parts of the body.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Excellent

I have found Creative Bioarray's cell products to be an excellent resource in my research.

03 Sep 2022

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For research use only. Not for any other purpose.