CAL-12T

LncRNA MALAT1 Promotes NSCLC Cells via Modulating MiR-200a-3p

Long non-coding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) promotes the progression of NSCLC by modulating miR-124 and miR-206. To further clarify the effect of MALAT1 and miR-200a-3p on the proliferation of NSCLC cells, MALAT1 plasmid was transfected into A549 cells to successfully establish MALAT1 overexpression models; MALAT1 shRNA was transfected into CAL-12T cells to successfully establish MALAT1 low expression models (Fig. 1a). miR-200a-3p mimics or inhibitors were also transfected into NSCLC cells, but they did not change the expression level of MALAT1 (Fig. 1b) and (Fig. 1c). Subsequently, a CCK8 assay was conducted. The proliferation rate of CAL-12T cells with low-expressed MALAT1 significantly decreased than that of the control group, and the proliferation of CAL-12T cells with lowly expressed MALAT1 significantly increased after transfection of miR-200a-3p inhibitors (Fig. 1d). Plate colony formation assays were performed to detect the proliferation of A549 and CAL-12T cells. The number of colonies of A549 cells with overexpressed MALAT1 significantly increased than that of the control group, and it significantly decreased after transfection of miR-200a-3p mimics. The number of colonies of CAL-12T cells with lowly expressed MALAT1 was significantly lower than that in the control group, and it significantly increased after transfection of miR-200a-3p inhibitors (Fig. 1e).

Fig. 1 LncRNA MALAT1 promotes the proliferation of NSCLC cells via modulating miR-200a-3p. (Wei S, et al., 2019)