ActoFactor™ Recombinant Rat Vascular endothelial growth factor 164

Specification

Cat.No.

CSC-CTK0841

Description

VEGF-164 is a polypeptide growth factor and a member of the platelet-derived growth factor family. It is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. Two high-affinity tyrosine kinase receptors for VEGF-164 have been identified, VEGFR-1 (FLT-1), and VEGFR-2 (Flk-1). Consistent with the endothelial cell-specific action of VEGF-164, expression of both receptor genes has been found predominantly but not exclusively on endothelial cells. Expression of VEGFR-1 was also found on human monocytes, neutrophils (PMNs), bovine brain pericytes and villous and extravillous trophoblasts. In addition to its action as a mitogen, it is a potent vascular permeability factor (VPF) in vivo and is also a chemo attractant for monocytes and endothelial cells. At least four different proteins are generated by differential splicing of the mouse VEGF gene: VEGF-120, VEGF-144, VEGF-164 and VEGF-188. The most abundant form is VEGF-164. Whereas VEGF-120, VEGF-144 and VEGF-164 are secreted proteins, VEGF-188 is strongly cell-associated. In addition, the isoforms VEGF-164 and VEGF-188 bind to heparin with high affinity. VEGF is apparently a homodimer, but preparations of VEGF show some heterogeneity on SDS gels depending of the secretion of different forms and the varying degrees of glycosylation. All dimeric forms possess similar biological activities. There is evidence that heterodimeric molecules between the different isoforms exists and that different cells and tissues express different VEGF isoforms. A related protein of VEGF is placenta growth factor (PlGF) with about 53% homology and VEGF-B with similar biological activities. The full ORF of native rat VEGF-164 (Ala27-Arg190) was cloned from total RNA of rat sinusoidal endothelial cells using standard protocols. Rat Vascular Endothelial Growth Factor164 (VEGF- 164), a 19.23 kDa protein consisting of 164 amino acid residues, is produced as a homodimer.

Species

Rat

Product Overview

Rat Vegfa expressed in E.coli

Molecular Mass

19.23 kDa

Size

CAT# CSC-CTK0841-5 (5 μg); CAT# CSC-CTK0841-25 (25 μg)

Expression System

E.coli

Purity

Greater than 95% as determined by SDS-PAGE analysis.

Activity

Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 2-10 ng/ml.

Endotoxin Level

Less than 1 EU/μg.

Formulation

Lyophilized from a steril-filtered protein solution in PBS without any stabilizers.

Reconstitution

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration not lower than 50 μg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

Storage & Stability

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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