ActoFactor™ Recombinant Rat Tumor necrosis factor

Specification

Cat.No.

CSC-CTK0834

Description

Recombinant Rat Tumor Necrosis Factor-alpha (rRa TNF-alpha) is a potent lymphoid factor, which exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. It is also involved in many other cellular events such as cell growth modulation, immune system regulation, autoimmune disease and inflammation. Creative Bioarray's TNF-alpha is a 17.3 kDa protein containing 157 amino acid residues.

Species

Rat

Product Overview

Rat Tnf expressed in E.coli

Molecular Mass

17.3 kDa

Size

CAT# CSC-CTK0834-20 (20 μg); CAT# CSC-CTK0834-100 (100 μg)

Expression System

E.coli

Purity

Greater than 97% as determined by SDS-PAGE and HPLC analysis.

Activity

The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is ≤ 0.05 ng/ml, corresponding to a specific activity of 2 x 10^7 units/mg.

Endotoxin Level

Less than 1 EU/μg.

Formulation

The protein was lyophilized from a protein solution in 20 mM phosphate buffer and 0.1 M NaCl.

Reconstitution

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

Storage & Stability

The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rRa TNF-alpha should be used immediately or stored long- term in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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