ActoFactor™ Recombinant Rat Interleukin 4

Specification

Cat.No.

CSC-CTK0818

Description

Recombinant Rat Interleukin-4 (rRa IL-4) is a potent lymphoid cell growth factor, which stimulates the growth and survivability of certain B and T cells. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. Recombinant rat IL-4 is a 14.0 kDa globular protein containing 126 amino acid residues. It has been purified by proprietary chromatographic techniques.

Species

Rat

Product Overview

Rat Il4 expressed in E.coli

Molecular Mass

14 kDa

Size

CAT# CSC-CTK0818-20 (20 μg); CAT# CSC-CTK0818-100 (100 μg)

Expression System

E.coli

Purity

Greater than 98% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.

Activity

Recombinant Rat IL-4 is fully biologically active when compared to a standard. Determined by its ability to suppress LPS-induced TNF-alpha and MIP-2 production in mouse splenocytes.

Endotoxin Level

Less than 1 EU/μg.

Formulation

Lyophilized from a sterile-filtered, concentrated protein solution with no additives.

Reconstitution

Please centrifuge product briefly before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

Storage & Stability

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rat IL-4 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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