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ActoFactor™ Recombinant Rat Interleukin 3
- Specification
Cat.No.
CSC-CTK0073
Description
Interleukin-3 (IL-3) is a species-specific pleiotropic cytokine produced primarily by activated T cells. IL-3 stimulates the proliferation and differentiation of hematopoietic stem cells, as well as various lineage-committed progenitors. The methionyl form of recombinant rat IL-3 contains 143 amino acids and has a
predicted molecular mass of approximately 16.3 kDa.
Species
Rat
Product Overview
Rat Il3 expressed in E.coli
Sequence
A DNA sequence corresponding to the mature rat IL-3 protein sequence (Ile 27 – Cys 169) was expressed in E. coli.
Size
CAT# CSC-CTK0073-25 (25 μg); CAT# CSC-CTK0073-125 (125 μg)
Expression System
E.coli
Purity
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity
The biological activity of recombinant rat IL-3 is measured in a cell proliferation assay using the factor-dependent mouse myeloblastic cell line, NFS-60. The ED50 is typically 4 - 16 ng/mL.
Endotoxin Level
Endotoxin level is <1.0 EU per 1 μg cytokine, as determined by the LAL method.
Formulation
Recombinant rat IL-3 is lyophilized from a 0.2 μm filtered solution in phosphate buffered saline (PBS) containing 50 μg bovine serum albumin per 1 μg cytokine.
Reconstitution
Centrifuge vial prior to opening. Reconstitute rat IL-3 at a concentration greater than 10 μg/mL with sterile PBS containing at least 0.1% human or bovine serum albumin.
Storage & Stability
Lyophilized rat IL-3 is stable for up to twelve months from date of receipt at -20°C to -70°C. Reconstituted rat IL-3 can be stored under sterile conditions at
2 - 8°C for one month, or at -20°C to -70°C (in a manual defrost freezer) for three months without detectable loss of activity. Avoid repeated freezing and thawing.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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