ActoFactor™ Recombinant Rat Interleukin 10

Specification

Cat.No.

CSC-CTK0823

Description

Recombinant Rat Interleukin-10 (rRa IL-10) is an immunosuppressive cytokine produced by a variety of mammalian cell types including macophages, monocytes, B-cells, T-cells and keratinocytes. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNF- alpha. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Recombinant Rat IL-10 is an 18.7 kDa protein of 160 amino acid residues.

Species

Rat

Product Overview

Rat Il10 expressed in E.coli

Molecular Mass

18.7 kDa

Size

CAT# CSC-CTK0823-10 (10 μg); CAT# CSC-CTK0823-50 (50 μg)

Expression System

E.coli

Purity

Greater than 95% as determined by SDS-PAGE and RP-HPLC analysis.

Activity

The ED50 as determined by the dose-dependent inhibition of MC/9 proliferation is < 10 ng/ml.

Endotoxin Level

Less than 1 EU/μg.

Formulation

Lyophilized from a sterile-filtered protein solution containing 25 mM sodium phosphate buffer (pH 7.25) and 100 mM NaCI.

Reconstitution

Please centrifuge product briefly before opening the vial. The lyophilized rRa IL-10 should be reconstituted in 5 mM Tris (pH 7.2) to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

Storage & Stability

The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rRa IL-10 should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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