ActoFactor™ Recombinant Rat Interleukin 1 beta

Specification

Cat.No.

CSC-CTK0814

Description

Recombinant Rat Interleukin-1beta (rRa IL-1 beta) is a potent immuno-modulator, which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. Rat IL-1 beta is a 17.3 kDa protein containing 153 amino acid residues.

Species

Rat

Product Overview

Rat Il1b expressed in E.coli

Molecular Mass

17.3 kDa

Size

CAT# CSC-CTK0814-10 (10 μg); CAT# CSC-CTK0814-50 (50 μg)

Expression System

E.coli

Purity

Greater than 97% as determined by SDS-PAGE and HPLC analysis.

Activity

The ED50 of Creative Bioarray's rat IL-1 beta as determined by the dose-dependent stimulation of murine D10S cells is less than 0.1 ng/ml corresponding to a specific activity of Greater than 1 x 10^7 units/mg.

Endotoxin Level

Less than 1 EU/μg.

Formulation

Lyophilized from a sterile-filtered protein solution in PBS (pH 7.4) with no additives.

Reconstitution

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultrapure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

Storage & Stability

The lyophilized IL-1 beta, though stable at room temperature for up to 3 weeks, is best stored desiccated below 0°C. Reconstituted rRa IL-1 beta should be used immediately or stored long- term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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