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ActoFactor™ Recombinant Rat Fibroblast growth factor 2
- Specification
Cat.No.
CSC-CTK0804
Description
FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4. Recombinant rat FGF-basic is a 16.3 kDa protein consisting of 145 amino acid residues.
Species
Rat
Product Overview
Rat Fgf2 expressed in E.coli
Molecular Mass
16.3 kDa
Size
CAT# CSC-CTK0804-50 (50 μg); CAT# CSC-CTK0804-250 (250 μg)
Expression System
E.coli
Purity
Greater than 95% as determined by SDS-PAGE and HPLC analysis.
Activity
The ED50 as determined by the dose-dependent stimulation of thymidine uptake by 3T3 cells is ≤ 0.2 ng/ml corresponding to a specific activity of Greater than 5 x 10^6 units/mg.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyophilized from a sterile-filtered solution with no additives.
Reconstitution
Please centrifuge product before opening vial. The lyophilized protein should be reconstituted with distilled, sterile water to a concentration of 0.1-1.0 mg/ml. For further dilution, carrier protein (0.1% BSA or HSA) should be added to avoid loss of bioactivity.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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