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ActoFactor™ Recombinant Rat Fat mass and obesity associated
- Specification
Cat.No.
CSC-CTK0838
Description
Receptor-associated Protein (aka RAP) averts the GCM-mediated enhancement of axon growth- ApoE-containing lipoproteins which are known to bind to receptors of the LDLr superfamily. In the presence of RAP, GCM doesn't increase the axon extension rate in relation to base medium. Furthermore, the addition of RAP to the base medium doesn't change the rate of axon extension. This implies that the growth stimulatory effect of apoE-containing lipoproteins secreted by glial cells is mediated by the LDLr family receptors. RAP annuls the growth stimulatory effect of GCM. Recombinant rat RAP produced in E. coli is a single, non-glycosylated polypeptide chain with a molecular mass of 38.86 kDa. It has been purified by proprietary chromatographic techniques.
Species
Rat
Product Overview
Rat FTO expressed in E.coli
Molecular Mass
38.9 kDa
Size
CAT# CSC-CTK0838-20 (20 μg); CAT# CSC-CTK0838-100 (100 μg)
Expression System
E.coli
Purity
Greater than 95% as determined by SDS-PAGE and HPLC analysis.
Activity
N/A
Endotoxin Level
Less than 1 EU/μg.
Formulation
White (freeze-dried) powder. Lyophilized from a sterile-filtered, concentrated protein solution in TBS (pH 7.5), 0.1% BSA and 0.09% NaN3.
Reconstitution
Please centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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