ActoFactor™ Recombinant Mouse Vascular endothelial cell growth factor 164

Specification

Cat.No.

CSC-CTK0722

Description

Vascular endothelial growth factor (VEGF) stimulates vascular permeability and actively mediates both angiogenesis and vasculogenesis in the fetus as well as the adult. It binds to transmembrane receptors (VEGF R1 and R2) on endothelial cells, thereby promoting endothelial proliferation, migration and survival, and inhibiting apoptosis. VEGF is required during embryogenesis and has a role in wound healing in adults. Alternately spliced isoforms of VEGF have been identified in the mouse, consisting of 120, 164 and 188 amino acids (aa). VEGF164 shares 97% aa sequence identity with the corresponding regions of rat and 89% with human, and its biological activities are not species-specific.biological activities are not species-specific. Recombinant mouse VEGF164 contains 164 amino acids and a 16 a.a. histidine-based tag for a total length of 180 a.a. and has a predicted molecular mass of 21.4 kDa including his-tag. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 30 kDa in SDS-PAGE. Recombinant mouse VEGF164 is produced in the endosperm tissue of barley grain (Hordeum vulgare), that exhibits up to 50 times less protease activity than E. coli or mammalian cells. Barley seed is void of any human or animal viral contaminants that could jeopardize your cell culture. Product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1 beta induction.

Species

Mouse

Product Overview

Mouse Vegfa expressed in Endosperm tissue of barley grain (Hordeum vulgare)

Molecular Mass

21.4 kDa

Size

CAT# CSC-CTK0722-10 (10 μg); CAT# CSC-CTK0722-50 (50 μg)

Expression System

Endosperm tissue of barley grain (Hordeum vulgare)

Purity

Greater than 98% as determined by SDS-PAGE gel analysis.

Activity

Bioactivity is assayed by measuring the dose dependent effect of mouse VEGF164 on proliferation of Human Umbilical Vein Endothelial Cells (HUVEC) cells. This batch of growth factor was tested by an independent company and exhibits an ED50 of 2.9 ng/ml corresponding to 0.34 x 10^6 U/mg specific activity, a value comparable to their in-house standard measured in parallel. Optimal concentration should be determined for specific applications and cell lines.

Endotoxin Level

Less than 0.05 EU/μg.

Formulation

Lyophlized from a sterile-filtered protein solution in PBS (pH 7.2).

Reconstitution

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0,1 - 1,0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

Storage & Stability

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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