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ActoFactor™ Recombinant Mouse Platelet derived growth factor-alpha
- Specification
Cat.No.
CSC-CTK0706
Description
PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGF is stored in platelet alpha-granules and released upon platelet activation. The PDGF is involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGF have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. Mouse recombinant PDGF-AA is a disulfide linked homodimeric, non-glycosylated, polypeptide chain containing 2 x 126 amino acids, having a total molecular mass of 28.9 kDa.
Species
Mouse
Product Overview
Mouse Pdgfa expressed in E.coli
Molecular Mass
28.9 kDa
Size
CAT# CSC-CTK0706-10 (10 μg); CAT# CSC-CTK0706-50 (50 μg)
Expression System
E.coli
Purity
Greater than 97% as determined by SDS-PAGE and RP-HPLC analysis.
Activity
Measured by the dose-dependent stimulation of Balb/c 3T3 cells proliferation. The expected ED50 for this effect is 8-10 ng/ml.
Endotoxin Level
N/A
Formulation
Sterile-filtered, white, lyophilized (freeze-dried) powder. Lyophilized from a concentrated (1 mg/ml) protein solution containing no additives.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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